Quercetin inhibits c-fos, heat shock protein, and glial fibrillary acidic protein expression in injured astrocytes

2000 ◽  
Vol 62 (5) ◽  
pp. 730-736 ◽  
Author(s):  
Bing Yi Wu ◽  
Albert Cheung Hoi Yu
Keyword(s):  
Heat Shock ◽  
Glial Fibrillary Acidic Protein ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Acidic Protein

Induction of heat shock protein 70 and glial fibrillary acidic protein in the postischemic gerbil hippocampus

1994 ◽  
Vol 9 (4) ◽  
pp. 369-375 ◽  
Author(s):  
Tsutomu Araki ◽  
Hiroyuki Kato ◽  
Xia-Hong Liu ◽  
Kyuya Kogure ◽  
Yasuo Itoyama
Keyword(s):  
Heat Shock ◽  
Glial Fibrillary Acidic Protein ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Acidic Protein

Expression of prostaglandin G/H synthase (PGHS) and heat shock protein-70 (HSP-70) in the corpus luteum (CL) of prostaglandin F2α-treated immature superovulated rats

2004 ◽  
Vol 82 (6) ◽  
pp. 363-371 ◽  
Author(s):  
R M Narayansingh ◽  
M Senchyna ◽  
M M Vijayan ◽  
J C Carlson
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Corpus Luteum ◽  
Heat Shock Protein 70 ◽  
Prostaglandin F2α ◽  
Sampling Period ◽  
Immunoblot Analysis ◽  
Hsp 70 ◽  
Prostaglandin F

In this study we examined the mechanism of corpus luteum (CL) regression by measuring changes in expression of prostaglandin G/H synthase-1 (PGHS-1) and -2 (PGHS-2) in day 4 CL and inducible heat shock protein 70 (HSP-70) in day 4 and day 9 CL of immature superovulated rats. The rats were superovulated and treated with 500 µg of prostaglandin F2α (PGF2α) on day 4 or day 9 after CL formation. Ovaries and serial blood samples were removed during the 24-hour period following treatment. Plasma progesterone was determined by radioimmunoassay while mRNA abundance and protein expression were assessed by semiquantitative RT-PCR and immunoblot analysis, respectively. One hour after PGF2α, both day 4 and day 9 rats exhibited a significant decrease in progesterone secretion; however, there was a greater decrease in day 9 rats. In ovarian samples removed on day 4, there was a significant increase in mRNA for PGHS-2 at 1 hour after PGF2α. PGHS-1 mRNA content remained unchanged. Immunoblot analyses showed an increase in PGHS-2 protein expression only at 8 h. There were no changes in PGHS-1 protein expression. In day 9 rats, ovarian HSP-70 protein levels increased by 50% after PGF2α injection; however, on day 4 there was no change in expression of this protein over the sampling period. These results suggest that expression of PGHS-2 may be involved in inhibiting progesterone production and that expression of HSP-70 may be required for complete CL regression in the rat.Key words: rat, prostaglandin F2α, corpus luteum, prostaglandin G/H synthase, heat shock protein-70.

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